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The assay mixture contained 1 M Tris, 5 mM EDTA buffer, pH 8.0, and 10 mM pyrogallol

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HRMS was developed in the 1960s with double-focusing mass spectrometers, but today, time-of-flight (TOF) or Orbitrap (OT) mass analyzers are common, mostly as hybrids with triple quadrupoles (QTOF, QOT) or ion traps in front, allowing fragmentation to reproducible MS/MS spectra [61]

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