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16S rDNA gene sequencing analysis Fresh ileum contents were collected for analysis, and specific primers with barcodes are used to amplify the V3 to V4 variable regions of the bacterial 16S rDNA via PCR

Whilst studies in humans have demonstrated that patients with asthma exhibit dysregulated rhythms in serum melatonin concentrations, the pathological mechanisms precipitated by this remain poorly understood ( Nfe2l2 was expressed in a rhythmic fashion