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After centrifugation at 14,000 rpm for 10 min at 4C to pellet cellular debris, supernatants were collected and protein concentrations determined using the DC protein assay kit (Bio-Rad)

Research in Archives of Biochemistry and Biophysics shows that TGF- decreases intracellular glutathione content in fibroblasts, triggering increased collagen I mRNA and protein production

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Secondly, it suppresses the release of glucagon, a hormone that raises blood sugar levels