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Discard the supernatant and resuspend the cells with stimulation buffer to a density of 0.6 million cells per mL, then add the cells to 384-well white plates (3,000 cells per well)

Synaptic co-release of thiamine and ACh, with thiamine facilitating the neurotransmission through its non-coenzyme action, is known from the early works of Minz and von Muralt on neuromuscular junctions (143, 165167)